Published on March 27, 2019

Today we bring you another perspective on how a life with multiple sclerosis (MS) can be improved with vitamin D. Three years ago, GrassrootsHealth checked in with Dr. Barbara M van Amerongen DDS, PhD on her personal experience and case study with MS and vitamin D. Below is a summary of her findings with an update on her latest interventions.

About Barbara M. van Amerongen, DDS, PhD

Dr. Barbara M. van Amerongen received her dental degree from the University of Amsterdam in 1974 and earned her PhD in 1985. At the University of Amsterdam, she was working at the Department of Preventive Dentistry; in addition she had her own part-time dental practice. In 1990 she was diagnosed with multiple sclerosis (MS), the symptoms having started around 1975. In 1997 she closed her dental practice, due to MS-related fatigue. She joined the Department of Molecular Cell Biology and Immunology, Amsterdam UMC, part of the MS Center, to study MS and Vitamin D. This resulted in two papers (van Amerongen et al., 2004 and Kragt et al., 2009). These papers led her to test her hypothesis, that vitamin D aids MS, on herself. In 2012, she published the findings of her self-case study, Effect of High-Dose Vitamin D3 Intake on Ambulation, Muscular Pain and Bone Mineral Density in a Woman with Multiple Sclerosis: A 10-Year Longitudinal Case Report (van Amerongen and Feron, 2012).

About Dr. van Amerongen’s Case Study

In January 2001 Dr. van Amerongen started with vitamin D3 800 IU/day, resulting in serum levels between 33-40 ng/ml (84-102 nmol/L). In September 2004 she increased to 4000 IU/day, which seemed to keep the serum level around 40 ng/ml (100 nmol/L). In December 2005 she increased again to 6000 IU/day, and serum levels rose to above 55 ng/ml (135 nmol/L). Although there were rises and falls in the serum level throughout the 10 year period, her vitamin D level was always within a “safe” physiological range of 30-80 ng/ml (75-200 nmol/L). She found it took her about 8 months at a new dosage level to reach a steady state 25(OH)D level. Calcium was also taken daily – initially 240 mg/day, and in the period from March to July 2009 increased to 1332 mg/day.

She used patient related outcome measures – ambulation (walking distance in km/day) and muscular pain (pain killers yes/no) – to keep track of her progress. In addition bone mineral density (BMD) and serum levels were monitored.


Ambulation was the key patient related outcome measure. The walking distance increased, as vitamin D levels increased, from 1 to 14 km/day.

(Chart note: for vitamin D3, 1 mcg/day = 40 IU/day)

Muscular pain decreased, as vitamin D levels increased. She stopped using a painkiller (Paracetamol). Even after a long walk, she no longer suffered from muscular pain, whether calf muscle pain or nocturnal leg cramps.

Unfortunately, her serum PTH level did not reach the lower limit of its normal reference range and her BMD decreased. She was diagnosed with osteopenia in 2009.

Adverse Effects

No adverse events including hypercalcaemia, nephrolithiasis or fracture, were observed. Serum calcium levels remained within the normal range. In January 2009, a scan with a sonographer revealed no renal calculus (kidney stones) after eight years of vitamin D supplementation. Additionally, no hypercalciuria was observed.

Discussion and Update — by Dr. van Amerongen
March, 2019

My paper discusses vitamin D resistance, the blunted parathyroid hormone (PTH) response, and ways to reduce the serum PTH level as found in the literature.

Vitamin D Deficiency & Vitamin D Resistance
Since the case study, I have increased my vitamin D3 intake from 150 mcg (6000 IU) to 250 mcg (10000 IU)/day. In addition I take magnesium instead of calcium supplements, as well as omega-3 (EPA/DHA), boron, resveratrol, vitamin B2 and K2.

Rule of thumb: “When the serum vitamin D level goes up, the serum PTH level goes down.”

After increasing the vitamin D3 intake from 6000 to 10000 IU/day the vitamin D level went up to above 60 ng/ml (150 nmol/L), but the PTH level remained the same, at the high end of its reference range. Dr. Coimbra calls this “vitamin D resistance” and it may be caused by one or more single nucleotide polymorphism (SNP) in genes involved in the vitamin D metabolic pathway (Coimbra 2018). Key genes of the vitamin D metabolic pathway (A-H in blue) are shown in fig 1 (Jolliffe 2016).

Fig. 1. A diagram depicting vitamin D metabolic and signaling pathways and genes encoding key players (A-H in blue). For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article. D.A. Jolliffe et al. / Journal of Steroid Biochemistry & Molecular Biology 164 (2016) 18-29 19

Coimbra regards the PTH level as the most valuable indicator of overall vitamin D resistance of an individual and uses the PTH level to adjust the required daily vitamin D3 dose. Coimbra’s aim is to keep the serum PTH level around the lower limit of its normal reference range. To test vitamin D resistance in people with autoimmune diseases, Coimbra uses a dose of vitamin D3 at 1000 IU/kg.


With a vitamin D3 intake of 10,000 IU/day I’m taking enough to nullify my vitamin D deficiency: a vitamin D level above 60 ng/ml, but I’m clearly not taking enough vitamin D3 to overcome my vitamin D resistance: a PTH level around the lower limit of its normal reference range as necessary according to the Coimbra Protocol (CP). A higher intake of vitamin D3 requires a CP doctor, the daily intake of 2.5 liters of water, a calcium free diet, daily exercise to prevent bone loss and stress management.

If you have an autoimmune disorder: Test your vitamin D, Calcium and PTH levels today.

More information

Van Amerongen publishes a web site with a FaceBook page (Vitamin D and MS) where she collects news on Vitamin D and MS, gives an overview of the ongoing clinical trials with Vitamin D in MS, and presents a growing number of researchers, including neurologists, interested in vitamin D and MS. There is also an outreach to Dutch people with MS.

How can I track my vitamin D intake and levels?

To help you track your vitamin D intake, status, and that of other nutrients, GrassrootsHealth has created an online tracking system called myData-myAnswers. This will help you know how your supplementation and intake may be affecting you and impacting your health. Check it out today!

How Can You Use this Information for YOUR Health?

Having and maintaining healthy vitamin D and other nutrient levels can help improve your health now and for your future. Measuring is the only way to make sure you are getting enough!

STEP 1 Order your at-home blood spot test kit to measure vitamin D and other nutrients of concern to you, such as omega-3s, magnesium, essential and toxic elements (zinc, copper, selenium, lead, cadmium, mercury); include hsCRP as a marker of inflammation or HbA1c for blood sugar health

STEP 2 Answer the online questionnaire as part of the GrassrootsHealth study

STEP 3 Using our educational materials and tools (such as our dose calculators), assess your results to determine if you are in your desired target range or if actions should be taken to get there

STEP 4 After 3-6 months of implementing your changes, re-test to see if you have achieved your target level(s)

Enroll in D*action and Build Your Custom Test Kit!